Isolation and characterization of bacteria from blowflies Chrysomya chloropyga collected from two different abattoirs in two communities

Abstract

The objective of this study was to isolate and identify bacteria found on the body surface of blowfly Chrysomya chloropyga collected from two different abattoirs. In this study, adult blowflies were obtained from two different abattoirs of two towns, the first set were collected from Iwo (Odori) abattoir while the second set were collected from Oluponna (Alaya) abattoir. Serial dilution of the whole-body homogenate was done to obtain the bacteria in them. The bacteria were sub cultured to get pure colony. These colonies were Gram stained and viewed under the microscope for identification. Several biochemical tests such as sugar test, starch hydrolysis, citrate test, MRVP test and, indole test were subsequently carried out. The sub culturing, Gram staining and ochemical tests were done to determine the possible organisms that could be isolated from blowflies. The organisms that were identified from Iwo abattoir were all Gram positive with Staphylococcus sp. (81.80%) being the highest in frequency, while those identified from Oluponna abattoir were a composition of both Gram positive and Gram negative bacteria with Bacillus sp. and Citrobacter sp. (32%) being the highest in frequency. This study however concludes that C. chloropyga had a wide range of bacteria on its body surface with Gram positive organisms being dominant as compared with gram negative, and Iwo abattoir blowflies had the highest number of pathogenic bacteria while Oluponna abattoir blowflies had the highest diversity of bacteria.