ir.bowen.edu.ng:8181/jspui/handle/123456789/286 These are publications by academic staff and postgraduate students of Microbiology programme 2026-04-15T17:58:32Z ir.bowen.edu.ng:8181/jspui/handle/123456789/3021 Title: BLY 306: Introduction to R stat 2023-01-01T00:00:00Z ir.bowen.edu.ng:8181/jspui/handle/123456789/3020 Title: BLY 302: Environmental biology 2023-01-01T00:00:00Z ir.bowen.edu.ng:8181/jspui/handle/123456789/2517 Title: Antifungal resistance pattern and phylogenetic relationship of fungi isolated from abattoir soil samples in Iwo, Osun State Authors: Ayoola, Philip Oluwatobi Abstract: Soil is the thin layer covering our planet earth, and is a very important resource for humans, animals, plants, and microorganisms. Many actions and initiatives to promote sustainable soil management and soil security have been undertaken by several institutions in many countries. Improper disposal of waste effluents from abattoirs have been documented to have effects on the soil properties and ultimately increasing the population of microflora, enteric and opportunistic pathogens. Soil-borne fungi are a major cause for different degrees of allergy and diseases in humans and animals. This study therefore aimed at isolation and characterization of fungal microbes associated with abattoir soil samples. Five (5g) of soil samples were collected each from four different abattoirs, Eleya, Odo-ori, Kaara and Adeke in Iwo, Osun State. One (1) gramme of each soil sample was weighed, serially diluted using sterile water, and then 1ml aliquots of 10-3 and 10-5 dilutions were cultured on sterile Sabouraud dextrose agar medium impregnated with streptomycin (0.2g) using pour plate method. Incubation was carried out at 25-27°C for 3days. Six (6) different fungal isolates were obtained and subjected to macroscopic and microscopic examinations. Antifungal susceptibility testing was also conducted using Kirby-Bauer disc diffusion technique. Molecular characterization of the fungal isolates by amplifying the internal spacer region (ITS) of ribosomal DNA using primers ITS1 and ITS4, gel electrophoresis using mid-range ladder and an expected amplicon between (600-800bp), with sequence blasting using NCBI BLAST tool. Phylogenetic relationships among the six (6) fungal isolates were determined by neighbor-joining method, using MEGA I I (Kimura 2- parameters) with 1000 bootstrap replication value. Result revealed that the isolates belong to five (5) different genera; Lasidioplodia, Geotrichum, Curvularia, Aspergillus, and Epicoccum, and percentage occurrences were 23.08%, 23.08%, 15.38%, 30.77%, and 7.69% respectively. Antifungal sensitivity test showed that all the isolates were susceptible to Ketoconazole (100% susceptibility), five (5) to Clotrimazole (76.92% sensitivity), three (3) to Amphotericin B (46.15% susceptibility), but none was susceptible to nystatin (0% sensitivity). Molecular characterization and phylogenetic relationships among the six (6) fungal isolates results revealed species of the fungi as Lasiodiplodia theobromae, Geotrichum candidum, Curvularia aerial, Aspergillus allahabadii, Aspergillus flavus, and Epicoccum sorghum, with percentage identity between 98.36% to 100%. The presence of these opportunistic or potentially pathogenic microorganisms in abattoir soil, and their possibility of toxin production pose a significant threat to humans , pre-harvest and post-harvest crops. They are indicators of a potential health risk to abattoir workers, residents near abattoirs, meat consumers, and consumers of crops grown on contaminated soil. The use of conventional antifungal drugs against invasive fungal infection is of great importance in clinical treatments. Molecular technique has shown reliability in characterization of isolates to species level. 2022-01-01T00:00:00Z ir.bowen.edu.ng:8181/jspui/handle/123456789/2515 Title: Comparative analysis of stool as an alternative sample in the diagnosis of pulmonary tuberculosis among adults and children in Ogbomoso, Nigeria Authors: Oladipo, Tunde Sunday Abstract: Tuberculosis (TB) is caused by Mycobacterium tuberculosis (MTB), it is a bacterial disease that is infectious which is spread by infectious droplet nuclei. It remains one of the top 10 causes of death in lower-income and lower-middle-income countries. In this study, a comparative analysis of stool samples from 101 adults (91 pre-confirmed pulmonary TB patients) and 99 children (presumptive TB patients). Their stool samples were collected, processed and analyzed using Xpert MTB/RIF Ultra technique and AFB microscopy. While using Xpert MTB/RIF Ultra assay as our “reference standard”, a sensitivity of 96%, Specificity of 83%, Positive Predictive Value (PPV) of 87%, and Negative Predictive Value (NNP) of 87% was observed with stool Xpert MTB/RIF Ultra technique. Also Ziehl Neelsen’s Stain Microscopy has a Sensitivity of 76%, Specificity of 23%, PPV of 100% and NNP of 49% while Fluorescence Stain Microscopy reveals a Sensitivity of 82%, Specificity of 75%, PPV of 92%, and NNP of 75% in stool sample. This study has shown that Xpert MTB/RIF Ultra assay in stool samples serves as an alternative to sputum in the diagnosis of pulmonary tuberculosis with high level of accuracy and precision. Furthermore, Fluorescence Stain Microscopy has shown to be more efficient than Ziehl Nelsen’s stain Microscopy. 2023-01-01T00:00:00Z